Adherent cells ks y 1 m21 hey t1 were harvested on the following day using a rubber policeman.
Rubber policeman sigma.
A policeman is a hand held flexible natural rubber or plastic scraper.
Natural rubber scrapers rod diam.
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Phosphate buffered saline ph 7 2 with mg ca pbs sigma magnesium chloride mgcl 2 calcium chloride cacl 2 sodium dodecyl sulfate sds tris base.
The instruments feature a flat.
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Viable cell counts were determined by trypan blue dye exclusion.
Hydrochloric acid hcl sodium hydroxide naoh ethylenediaminetetraacetic acid edta dithiothreitol dtt sodium chloride.
Blunt tapered or round spatula tips in any size are designed to not scratch or damage surface when handling.
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Cells were grown to 70 confluency in 150 mm dishes after which tgf β1 was added at a final concentration of 100 pm.
A policeman also comes in various sizes shapes and types.
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The common type of it is attached to a glass rod and used in chemical laboratories to transfer residues of precipitate or solid on glass surfaces when performing gravimetric analysis this equipment works well under gentle delicate and precise requirement.
The versatile double ended styles have two different shaped blades for precise tailoring to specific testing applications.
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The cells were scraped with a sterile rubber policeman sigma aldrich collected in pbs in 1 5 ml tubes eppendorf and stored at 80 c.
Each cell pellet was lysed in 112 5 μl of lysis buffer 10 mm tris ph 8 0 10 mm edta ph 8 0 1 np 40 1 mm pmsf for 20 min at 4 c diluted with 634 5 μl of dilution buffer 10 mm tris ph 8 0 and.
Ideal for scraping and mixing transfer collect or stir biological samples with complete ease by using the multi purpose spatulas.
Qiashredder spin columns qiagen 3.
Cells grown in suspension u937 hl 60 a6876 p3hr1 and adherent cells were transferred to 12 15 mm round bottomed centrifuge tubes.
